Figure 3.
Determining the application limits of Plant_SNP_TATA_Z-tester on experimental data about tobacco development in the dark or under light [53], which indicated that the inverse problem under study is ill-posed. Legend: see the legend of Figure 1. (a) The result of our Web service Plant_SNP_TATA_Z-tester created in this work in the case of the comparison between the prototype Pmec of the artificial promoter for plant genes (the textbox “1st promoter”) and its mutant variant “G13c” (the textbox “2nd promoter”). (b,c) Statistically significant correlations between the in silico predicted −ln(KD) values of TBP–promoter affinity expressed in ln units, which characterize the complexes formed by tobacco TBP binding to various artificial promoters based on the Pmec prototype [59] (x-axis) and the in vivo efficiency magnitudes of the reporter gene gusA expression (y-axis) on tobacco development in the dark or under light, respectively.