FIG. 1.

Scheme of the yeast genomic DNA region containing JEN1 and Southern blot analysis of its disruption. (a) Schematic restriction map of the yeast genomic DNA fragment contained in the plasmid pT12. This plasmid, which contains the complete sequence of JEN1 (YKL 217w), was isolated from the original YEp13 genomic library. (b) Disruption of JEN1 was carried out as described in the text. The 2.7-kb SacI-XbaI fragment was used to replace the genomic wild-type copy. (c) Southern blot analysis of the JEN1 disruption. Genomic DNA was digested with HindIII, subjected to electrophoresis in a 0.8% agarose gel, transferred to Hybond-N membrane, and hybridized with the 855-bp probe indicated in panels a and b. Lanes: 1, DNA from wild type; 2, DNA from BLC 203 strain carrying the jen1::HIS3 disruption. Sizes of the fragments are indicated at left.