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. 2022 Jul 29;23(15):8380. doi: 10.3390/ijms23158380

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Cytotoxic effect of liposome-PEG and liposome-PEG-CP7 on HFL-1 cells. (b) Cytotoxic effect of siMcl-1/liposome-PEG-CP7 on A549 cells. (c) Cell uptake of siRNA/liposomes on A549 cells determined with FCM. (d) Cell uptake and intracellular distribution of siRNA/liposomes in A549 cells determined with CLSM. (e) Apoptosis induction by siRNA/liposomes in A549 cells determined with FCM. (f) Level of Mcl-1 protein expressed in A549 cells treated with siRNA/liposomes determined with Western blot technique. (g) Level of Mcl-1 mRNA expressed in A549 cells treated with siRNA/liposomes determined with RT−PCR. *: differences between siMcl-1/liposome-PEG-CP7 with other groups, ***: p < 0.001, ****: p < 0.0001.