Figure 7.

Silencing of ShROP7 reduced defense responses in tomato following powdery mildew infection. (A) Histological observation of H₂O₂ accumulation in CK and ShROP7-silenced plants inoculated with On-LZ at 24, 48 and 72 hpi in LA1777 plants. (B) The H₂O₂ production rate was calculated at 6, 18, 24, 48 and 72 hpi. (C) Histological observation of hypersensitive cell death in TRV2 and ShROP7-silenced plants inoculated with On-LZ at 24, 48 and 72 hpi in LA1777 plants. Blue (trypan) staining indicates hypersensitive cell death. (D) The HR production rate was calculated at 6, 18, 24, 48, and 72 hpi. Error bars represent the variations among three independent replicates. Asterisks (**) indicates statistically significant differences between TRV2 and TRV2::ShROP7 plants at each time point (p < 0.01). Co, conidium; App, appressorium; Agt, appressorium germ tube; Sh, secondary hyphae; Ha, haustorium; Sa, secondary appressorium; HR, hypersensitive response. Bar, 50 μm.