Table 1.

Protein interactions of ShROP7 identified from yeast two-hybrid screen.

Strain No.	Interacting Target Proteins	Accession No.	Description
1	Calcium-binding EF-hand family protein	Solyc03g117470.2.1	Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins.
2	60S ribosomal protein L26-1	Solyc02g064670.1.1	Ribosomal large subunit biogenesis; cytoplasmic translation.
3	eukaryotic initiation factor 4A-2	Solyc08g062800.2.1	ATP-dependent RNA helicase activity; RNA secondary structure unwinding; translational initiation.
4	Uncharacterized protein	Solyc05g009220.2.1	Glutamine-tRNA ligase; glutaminyl-tRNA synthetase; belongs to the class-I aminoacyl-tRNA synthetase family.
5	40S ribosomal protein S20-2	Solyc01g096580.2.1	Structural constituent of ribosome; cytoplasmic translation.
6	PGH1	Solyc09g009020.2.1	Magnesium ion binding; phosphopyruvate hydratase activity; glycolytic process.
7	Uncharacterized protein	Solyc07g007680.2.1	Membrane contacts sites (MCSs) domains are exclusively found at MCSs between different organelles; mediates lipid transfer between the two adjacent bilayers independently of membrane fusion and fission reactions.
8	proteasome subunit alpha type-3	Solyc10g081130.1.1	Proteasome subunit alpha type; the proteasome is a multicatalytic proteinase complex.
9	ranslocase of chloroplast34	Solyc03g095220.2.1	GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis.
10	U3 small nucleolar ribonucleoprotein	Solyc01g007250.2.1	Some proteins in this family suggest a role in ribosome biogenesis and rRNA binding.
11	ERAD-associated E3 ubiquitin-protein ligase component HRD3A	Solyc03g118670.2.1	It mediates protein-protein interactions and the assembly of multiprotein complexes.
12	wound-induced proteinase inhibitor 1	Solyc09g084440.2.1	The proteinase inhibitors inhibit peptidases of the S1 and S8 families; synthesis of the inhibitors throughout the plant is also induced by leaf damage.
13	exonuclease family protein	Solyc01g096570.2.1	Ribonuclease T is responsible for the end-turnover of tRNA and removes the terminal AMP residue from uncharged tRNA.
14	Bap31	Solyc02g032930.2.1	Play a role in endoplasmic reticulum (ER) quality control and sorting
15	Fructose-bisphosphate aldolase	Solyc05g008600.2.1	It is a glycolytic enzyme that catalyzes the reversible aldol cleavage or condensation of fructose-1,6-bisphosphate into dihydroxyacetone- phosphate and glyceraldehyde 3-phosphate.
16	Putative PTI1-like tyrosine-protein kinase 3	Solyc12g098820.1.1	Tyrosine-protein kinases can transfer a phosphate group from ATP to a tyrosine residue in a protein.
17	5-oxoprolinase	Solyc09g010560.1.1	Participate in glutathione metabolism.
18	EIL1	Solyc06g073720.1.1	DNA-binding transcription factor activity; cellular response to iron ion; ethylene-activated signaling pathway.
19	OxaA/YidC-like membrane insertion protein	Solyc05g014050.2.1	Required for the insertion of some light harvesting chlorophyll-binding proteins (LHCP) into the chloroplast thylakoid membrane.
20	Ferredoxin	Solyc10g075160.1.1	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
21	Thioredoxin	Solyc06g060290.2.1	Thioredoxin serves as a general protein disulphide oxidoreductase. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide.
22	Cellulose synthase	Solyc01g087210.2.1	This protein is involved in the pathway plant cellulose biosynthesis.
23	Pectin acetylesterase (PAEs)	Solyc08g005800.2.1	PAEs catalyze the deacetylation of pectin, a major compound of primary cell walls.
24	60S ribosomal protein L24	Solyc09g008800.2.1	L24e/L24 appears to play a role in the kinetics of peptide synthesis and may be involved in interactions between the large and small subunits, either directly or through other factors.
25	Glutathione peroxidase (GSHPx)	Solyc08g006720.2.1	GSHPx is an enzyme that catalyzes the reduction of hydroxyperoxides by glutathione. Its main function is to protect against the damaging effect of endogenously formed hydroxyperoxides.
26	15.7 kDa heat shock protein	Solyc04g014480.2.1	These seem to act as chaperones that can protect other proteins against heat-induced denaturation and aggregation.
27	vesicle transport v-SNARE 12	Solyc05g013050.2.1	SNAP receptor activity; vesicle-mediated transport; intracellular protein transport; V-SNARE proteins are required for protein traffic between eukaryotic organelles.
28	Serinethreonine-protein kinase sty46	Solyc10g055720.1.1	Protein kinases catalyze the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function.
29	DAR GTPase 3	Solyc06g084270.2.1	GTP binding; GTPase activity
30	MAPK16	Solyc12g040680.1.1	Eukaryotic serine-threonine mitogen-activated protein (MAP) kinases are key regulators of cellular signal transduction systems and are conserved; MAPKs play important roles in the signaling of most plant hormones and in developmental processes
31	F-box protein At5g46170	Solyc08g048430.2.1	The F-box is a conserved domain that is present in numerous proteins with a bipartite structure. Through the F-box, these proteins are linked to the Skp1 protein and the core of SCFs (Skp1-cullin-F-box protein ligase) complexes. SCFs complexes constitute a new class of E3 ligases.
32	D111/G-patch domain-containing protein	Solyc08g081580.2.1	It might be a previously undetected RNA-binding domain mediating a distinct type of RNA-protein interaction.
33	Uncharacterized protein	Solyc08g080110.2.1	It is deubiquitinating (DUB) enzymes known as the MINDY family (MIU-containing novel DUB).
34	CHI3	Solyc02g082920.3	Defense against chitinase activity; cell wall macromolecule catabolic process; chitin catabolic process; defense response; polysaccharide catabolic process.
35	GATA transcription factor 5	Solyc01g110310.2.1	Transcriptional activator that specifically binds 5′-GATA-3′ or 5′-GAT-3′ motifs within gene promoters; positive regulation of transcription.