FIG. 2.

Southern blot of inverse PCR fragments. Chromosomal DNA was digested with HhaI and self-ligated. An inverse PCR was performed using 5′-GGT GAG GTA ACG GCT CA-3′ as the forward primer and 5′-GGG TCC ATC CAT AAG TGA-3′ as the reverse primer. The PCR products were separated on an agarose gel and blotted onto nitrocellulase. A Southern analysis using DIG-labelled oligonucleotide probes directed against the psychrotolerant (GAT AAT ATT TTG AAC TGC ATA G) and the mesophilic (GAT AAC ATT TTG AAC CGC ATG G) signature 1 was performed. The experiment was done two or three times, and one of the identical experiments is presented. Lanes: 1, WSBC10028; 2, WSBC10250; 3, WSBC10246; 4, WSBC10316; 5, HER1410; 6, WSBC10310; 7, WSBC10314; 8, WSBC10297; 9, WSBC10311; 10, WSBC10204. Left lane of each pair of blots, psychrotolerant probe; right lane, mesophilic probe.