FIG. 3.

Correlation of the psychrotolerance indices obtained by restriction digest (Fig. 1) and inverse PCR (Fig. 2). The proportion of psychrotolerant signatures from the inverse PCR is determined as a percentage of those bands that appear predominantly with the psychrotolerant probe. These data are plotted on the abscissa. The intensity of the bands resulting from the SspI restriction was measured with the Imagemaster 1D software package. The proportion of the psychrotolerant (351-bp) band is expressed as a percentage of the total PCR product (the 351-bp band plus the 473-bp band). These data are plotted on the ordinate. The experiment was done twice, and the means of the populations were determined. Standard errors were less than 10%.