Skip to main content
. 2022 Aug 3;23(15):8645. doi: 10.3390/ijms23158645

Figure 4.

Figure 4

ACY-241 and PCI-34051 treatment synergistically suppresses cell migration. (A,B) Immunoblotting of TWIST1, MMP-9, and ZEB1 in TOV-21G and A2780 cells treated with 3 µM ACY-241 and 20 µM PCI-34051 alone or in combination for 24 h. Protein expression levels were semi-quantified relative to the loading control, α-tubulin. (C,E) Transwell migration assay in TOV-21G and A2780. Cells were treated with 3 µM ACY-241 and 20 µM PCI-34051 alone or in combination for 48 h. Migrated cells were stained and photographed at 100× magnification. (D,F) Wound healing assay in TOV-21G and A2780. Cells were treated with 3 µM ACY-241 and 20 µM PCI-34051 alone or in combination for 24 h. The width of the scratch was calculated and photographed at 50× magnification. Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, or *** p < 0.001 vs. DMSO control; # p < 0.05. ## p < 0.01, or ### p < 0.001 vs. ACY-241-treated group; $ p < 0.05, $$ p < 0.01, or $$$ p < 0.001 vs. PCI-34051-treated group.