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. 2022 Aug 3;23(15):8645. doi: 10.3390/ijms23158645

Figure 5.

Figure 5

ACY-241 and PCI-34051 treatment synergistically enhances p53 stability in p53 wild-type ovarian cancer cells. (A) Immunoblotting of acetyl-p53. Cells were treated with indicated doses of PCI-34051 for 24 h. (B,C) Immunoblotting of acetyl-p53 and p21. Cells were treated with 3 µM ACY-241 and 20 µM PCI-34051 alone or in combination for 24 h. Protein expression levels were semi-quantified relative to the loading control, α-tubulin. Relative protein levels of acetyl-p53 were semi-quantified relative to total p53 levels and relative protein levels of acetyl SMC3 were semi-quantified relative to total SMC3 levels. Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, or *** p < 0.001 vs. DMSO control; ## p < 0.01, or ### p < 0.001 vs. ACY-241-treated group; $ p < 0.05, $$ p < 0.01, or $$$ p < 0.001 vs. PCI-34051-treated group.