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. 2022 Aug 4;23(15):8688. doi: 10.3390/ijms23158688

Figure 8.

Figure 8

No effect of combined peptides pGRP and pCIB on collagen-induced platelet aggregation or Ca2+ fluxes. (A,B) Platelet preparations (250 × 108/L) were pre-incubated with vehicle control, tirofiban (1 μg/mL) or indicated peptides (50 μg/mL) for 10 min. Platelet aggregation was monitored by light transmission aggregometry in response to 1 μg/mL collagen I or (i) or 1 μg/mL CRP-XL (ii). (A) representative aggregation traces (B) and normalized transmission changes. (C) Fura-2-loaded platelets were pre-incubated with vehicle control, tirofiban (1 μg/mL), or indicated peptides (50 μg/mL) for 10 min, before addition to 96-well plates. After supplementation of 1 mM CaCl2, loaded platelets were automatically stimulated with 10 μg/mL collagen I (i) or 10 μg/mL CRP-XL (ii). Dual wavelength 340/380 nm fluorescence changes per well were recorded in a FlexStation 3. Shown are representative [Ca2+]i traces per agonist. Mean values ± SD (n = 3 donors); n.s., not significant, * p < 0.05, ** p < 0.005, *** p < 0.001, vs. vehicle (paired Student’s t-test).