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. 2022 Aug 8;23(15):8798. doi: 10.3390/ijms23158798

Figure 2.

Figure 2

Akt in mediating the effect of PEBP4 on mTORC1, but not mTORC2. (A) The MHCC97H cells containing sh-PEBP4 or sh-NC were infected with adenovirus encoding the active Akt1 mutant (Akt-S473D) or GFP as a control at different doses and Western blots carried out using antibodies, as indicated. (B,C) The immunoblotted bands were analyzed by densitometry, as in Figure 1. (D,G) The MHCC97L cells containing pCMV6-PEBP4 or pCMV6-NC were treated with or without MK-2206 (5 µM, 24 h), an Akt specific inhibitor, and extracted blotted with antibodies. (E,F,H,I) Quantification of bands in (D,G) was performed. Results were presented as mean ± SEM (n = 3). Significant differences were examined by student’s t-test. * p < 0.05, ** p < 0.01, ## p < 0.01, && p < 0.01, ϕϕ p < 0.01.