Figure 6.

The role of Akt in mediating the effect of PEBP4 on proliferation, migration, invasion, and EMT markers of HCC. (A–D) MHCC97H cells containing sh-PEBP4 or sh-NC were infected with adenovirus expressing Akt S473D or GFP. Cell proliferation (A), migration and invasion were assayed (B) and quantified (C,D). (E,F) EMT markers were examined by Western blot € and WB bands semi-quantified as opposed to actin (F). (G–L) MHCC97L cells transfected with pCMV6-PEBP4 or the empty vector pCMV6-NC were treated with MK2206 or DMSO, cell proliferation (G), cell migration, and invasion were examined (H) and quantified (I,J). EMT markers were examined by WB (K) and bands were quantified against actin (L). All experiments were repeated three times. Data were presented as mean ± SEM (n = 3), ** p < 0.01, ^## p < 0.01, ^ϕϕ p < 0.01.