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Exogenous ANP treatment ameliorated myocardial insulin resistance. In vivo myocardial insulin sensitivity was assessed by measuring insulin-induced phosphorylation of Akt. (A) Representative immunoblots for phospho- and total Akt from cardiac lysates. (B) Densitometric quantitation normalized to the level of p-Akt/total Akt expression in HFD with insulin hearts is shown (n = 5 each). * p < 0.05 versus HFD, † p < 0.05 versus HFD + ANP, ‡ p < 0.05 versus NFD, § p < 0.05 versus NFD+ANP.
