Figure 1.

Analysis of ANG expression level in HaCaT cells cultured in different growth conditions. (A). Expression analysis of ANG mRNA by RT-qPCR; (B). Western blot analysis of cell lysates. CTRL: cells cultured in physiological conditions; SA: HaCaT cells treated with 500 µM sodium arsenite (SA) for 1 h at 37 °C; H₂O₂: HaCaT cells treated with 1 mM of hydrogen peroxide for 2 h at 37 °C; T₄₅: HaCaT cells subjected to thermal stress at 45 °C for 1 h.