Figure 8.

(A) Nomifensine (Nomif) reversed the (±)methamphetamine (methamph)-induced [³H]dopamine release from rat striatum. For the experimental procedure, see Figure 2B. (±)Methamphetamine was added to striatal slices from fraction 10 in a concentration of 6.75 µmol/L, nomifensine was added to the slices from fraction 5 in a concentration of 10 µmol/L and the drugs were maintained throughout the experiment. The (±)methamphetamine-induced [³H]dopamine release was 12.73 ± 2.20 and nomifensine decreased this release to 1.15 ± 0.26 percent of content (p < 0.01). Student t-statistics for two means, mean ± S.E.M., n = 4-4. (B) Nomifensine inhibited (±)methamphetamine-induced non-vesicular [³H]dopamine release in a concentration-dependent manner. (±)Methamphetamine was added to striatal slices from fraction 10 in a concentration of 6.75 µmol/L, nomifensine was added to the slices in a concentration of 1 or 10 µmol/L from fraction 1 and the drugs were maintained throughout the experiment. One-way ANOVA followed by the Dunnett’s test, F(3,12) = 15.58, p = 0.0002, * p < 0.05 mean ± S.E.M., n = 4.