Figure 7.

Effects of siRNA knockdown on the expression of vtgs and ers in primary hepatocytes: (A–F) the mRNA levels of vtgaa, vtgab, vtgc, erα, erβ1 and erβ2 in the hepatocytes. The cells were transfected with si217 or si353 (5 μg /mL), and then treated with 0.01 μM E₂ for 24 h. Data are expressed as the mean ± SEM (n = 4) and analyzed by one-way ANOVA followed by Tukey’s post-hoc test. Different letters above the error bars indicate statistical differences at p < 0.05.