Table 3.
Primers used for rapid amplification, site-directed mutagenesis, and plasmid construction.
Gene | Primer Name | 5′ to 3′ Sequence |
---|---|---|
Primers used for rapid amplification of cDNA ends | ||
erα | erα-race-R1 | AGTCATTGTGACCCTGAATGCTCC |
erα-race-R2 | CATACTGTATGCCTCGTCACTG | |
Primers used for Site-directed mutagenesis | ||
erα | Mut-sp1-F | CCACCtcgataaagCATTTGGGATTGATTGTGTAATTATTG |
Mut-sp1-R | ATGctttatcgaGGTGGCTATTTTAATCAGATGCTG | |
erα | Mut-ERE1-F | GAGATTgacttcCCTGCTTTGTTTGCTGTGTTATG |
Mut-ERE1-R | AGCAGGgaagtcAATCTCATTCCAACAGCCAATAATT | |
erα | Mut-ERE2-F | TTATGGgacttcGGCAGTAAAACACACTGCTGTTTG |
Mut-ERE2-R | ACTGCCgaagtcCCATAACACAGCAAACAAAGCAG | |
Primers used for plasmid construction | ||
erα | erα-1434-F | TGGCCTAACTGGCCGGTACCGAGCTCCTGTTGTAACTGGT |
erα-1434-R | TCTTGATATCCTCGAGGTCACTGAAGGGGGCACGA | |
erα-1057-F | TGGCCTAACTGGCCGGTACCCAATGAAATGTCATGAGG | |
erα-825-F | TGGCCTAACTGGCCGGTACCGCATCTTTAATTTGTTTATC | |
erα-555-F | TGGCCTAACTGGCCGGTACCTTACTGCAGAGTCTCAGG | |
erβ2 | erβ2-ORF-F | CTAGCTAGCATGGCCTCGTCCCCTGAGCT |
erβ2-ORF-R | CCGGAATTCCTACTGGTTCCACTGATGGA |