Table 3.
Primers used for rapid amplification, site-directed mutagenesis, and plasmid construction.
| Gene | Primer Name | 5′ to 3′ Sequence |
|---|---|---|
| Primers used for rapid amplification of cDNA ends | ||
| erα | erα-race-R1 | AGTCATTGTGACCCTGAATGCTCC |
| erα-race-R2 | CATACTGTATGCCTCGTCACTG | |
| Primers used for Site-directed mutagenesis | ||
| erα | Mut-sp1-F | CCACCtcgataaagCATTTGGGATTGATTGTGTAATTATTG |
| Mut-sp1-R | ATGctttatcgaGGTGGCTATTTTAATCAGATGCTG | |
| erα | Mut-ERE1-F | GAGATTgacttcCCTGCTTTGTTTGCTGTGTTATG |
| Mut-ERE1-R | AGCAGGgaagtcAATCTCATTCCAACAGCCAATAATT | |
| erα | Mut-ERE2-F | TTATGGgacttcGGCAGTAAAACACACTGCTGTTTG |
| Mut-ERE2-R | ACTGCCgaagtcCCATAACACAGCAAACAAAGCAG | |
| Primers used for plasmid construction | ||
| erα | erα-1434-F | TGGCCTAACTGGCCGGTACCGAGCTCCTGTTGTAACTGGT |
| erα-1434-R | TCTTGATATCCTCGAGGTCACTGAAGGGGGCACGA | |
| erα-1057-F | TGGCCTAACTGGCCGGTACCCAATGAAATGTCATGAGG | |
| erα-825-F | TGGCCTAACTGGCCGGTACCGCATCTTTAATTTGTTTATC | |
| erα-555-F | TGGCCTAACTGGCCGGTACCTTACTGCAGAGTCTCAGG | |
| erβ2 | erβ2-ORF-F | CTAGCTAGCATGGCCTCGTCCCCTGAGCT |
| erβ2-ORF-R | CCGGAATTCCTACTGGTTCCACTGATGGA | |