Figure 1.

The effects of osteostatin on osteoclast differentiation. Osteoclast precursors were stimulated with M-CSF and RANKL for osteoclast differentiation in the presence or absence of osteostatin (final concentrations: 100, 250 and 500 nM) for 7–9 days. Cells were TRAP and hematoxylin stained, and TRAP⁺ positive cells with 3 or more nuclei were counted under a light microscope. (A) Representative images; Bar = 100 μm. (B) TRAP⁺ multinucleated cells (MNCs) per well are expressed as the mean ± S.D. of three independent experiments; ** p < 0.01 vs. M-CSF+RANKL.