Figure A3.

The AG in a serum-free medium strongly reduces seminal plasma-derived cytotoxic effects in primary vaginal tissue. Here, 2 × 2 × 1 mm³ vaginal tissue blocks derived from four individual donors were incubated with 10% or 20% seminal plasma (or buffer) in the presence of a chemically defined, serum-free medium (X-vivo15) supplemented with 0.05 mM AG or in the presence of ECM growth medium supplemented with 10% FCS. After 3 days, the individual blocks were washed twice with PBS and analyzed using a CellTiter-Glo™ viability assay. Here, 0.5% triton was used as a toxic control. Black lines indicate averages, and RLU/s is defined as relative light units per second.