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. 2022 Jul 31;23(15):8510. doi: 10.3390/ijms23158510

Figure 1.

Figure 1

Emodin and vinblastine decrease viability and induce apoptosis in HeLa cells. Cells treated for 24 h with 40 and 80 µM concentrations of emodin, vinblastine (10 µM), and the combined action of 10 µM vinblastine with emodin at concentrations of 40 and 80 µM. (A) Effect of emodin and vinblastine on cell viability as assessed by MTT test. (B) Level of apoptosis assessed by annexin V-PE/7-AAD staining. Live cells (annexin V-PE-/7-AAD-), cells in early- (annexin V-PE+/7-AAD-) and late-stage apoptosis (annexin V-PE+/7-AAD+), and dead cells (annexin V-PE-/7-AAD+). (C) Percentage of apoptotic cells induced by tested agents. Differences statistically confirmed at: *** p < 0.001.