Figure 5.

The INS splice isoform inhibits growth rate and activates endoplasmic reticulum stress response in MIN6 insulinoma cells. (A) Proliferation rate of MIN6 cells transfected with the mutant and wild type INS cDNA sequences at days 6 and 13 after transfection. (B) Activation of luciferase gene under control of ER stress response element (ERSE) (left panel), unfolded protein response element (UPRE) (middle panel) or GRP78/BiP promoter (right panel) in MIN6 cells in the presence of phCMV-preproIns wild type and SNP are shown. The data are presented as means ± SD (n = 3), * p < 0.05, ** p < 0.01. All results are normalized to the activity of β-galactosidase.