TABLE 1.
Effect of external cAMP on the derepression of different enzymes
| Enzyme | Activity (nmol/min/mg of protein)a
|
|
|---|---|---|
| Ethanol | Ethanol + 5 mM cAMPb | |
| FbPase | 61 ± 6 | 2 ± 1 (≤1) |
| Phosphoenolpyruvate carboxykinase | 270 ± 86 | 43 ± 15 (20) |
| Isocitrate lyase | 120 ± 22 | 32 ± 13 (≤1) |
| NAD-dependent glutamate dehydrogenase | 210 ± 23 | 90 ± 27 (10) |
a
Enzymatic activities were measured in extracts of S. cerevisiae OL556 (pde2) after 12 h of derepression on ethanol in the presence or absence of cAMP, as described in the text. Values are averages and standard deviations of at least six independent experiments.
b
Activity in repressed cells is indicated in parentheses.