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. 2022 Jul 28;27(15):4834. doi: 10.3390/molecules27154834

Figure 8.

Figure 8

Pinus mugo EO inhibits cell migration in DU145 cells. (a) DU145 cells were treated with 25 and 50 μg/mL PMEO for the indicated time points. (b) % Wound closure was calculated using ImageJ/Fiji software (https://imagej.net/Fiji). (c) DU145 cells were treated with 25, 50 and 75 μg/mL PMEO for 24 h, and total protein extracts were analyzed by western blot using anti-ZEB1, anti-TWIST-1 and anti-Vimentin antibodies. Β-Actin was used as the internal loading control. The data shown are representative of four independent experiments.