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The representative example of overlaid, dynamic meibomian images (DMI) using Fiji. (A) Anonymized DMI of lower eyelid before mechanical expression. (B) Binary mask images constructed from A, by manual delineation of each meibomian glands. (C) Anonymized DMI of lower eyelid after mechanical expression. (D) Overlay of the mask image of pre-expression (B) onto the post-expression DMI image (C).
