Table 4.

Community compositions and relative abundance of phyllosphere microorganism from tobacco leaves infected by Alternaria alternata at bacterial phyla and genera levels (%).

Leaf type	Collecting time	Leaf position	Sample name	Phyla					Genera
				Proteobacteria	Firmicutes	Actinobacteriota	Bacteroidota	Pseudomonas	Pantoea	Kosakonia	Ralstonia	Sphingomonas	Methylobacterium-Methylorubrum
Healthy group	First time	Up	BJY871U	79.17 ± 18.04abc	12.50 ± 10.83abc	4.17 ± 3.61a	0.00 ± 0.00b	14.58 ± 20.09de	10.42 ± 18.04ab	6.25 ± 10.83c	4.17 ± 7.22ab	16.67 ± 28.87a	14.58 ± 15.73ab
		Middle	BJY871M	75.00 ± 22.53abcd	16.67 ± 18.04abc	2.08 ± 3.61a	2.08 ± 3.61ab	35.42 ± 30.83bcde	2.08 ± 3.61b	2.08 ± 3.61c	12.50 ± 6.25ab	12.50 ± 6.25a	10.42 ± 9.55ab
		Lower	BJY871D	52.08 ± 37.67cd	31.25 ± 28.64ab	0.00 ± 0.00a	6.25 ± 0.00a	8.33 ± 7.22de	6.25 ± 10.83ab	10.42 ± 13.01bc	2.08 ± 3.61ab	8.33 ± 9.55a	6.25 ± 0.00ab
	Second time	Up	BJY872U	62.50 ± 16.54bcd	18.75 ± 27.24abc	8.33 ± 14.43a	6.25 ± 6.25a	10.42 ± 13.01de	0.00 ± 0.00b	0.00 ± 0.00c	16.67 ± 28.87ab	16.67 ± 18.04a	6.25 ± 10.83ab
		Middle	BJY872M	52.08 ± 29.54cd	37.50 ± 21.65a	6.25 ± 6.25a	2.08 ± 3.61ab	27.08 ± 25.26de	4.17 ± 7.22ab	4.17 ± 3.61c	4.17 ± 3.61ab	12.50 ± 6.25a	0.00 ± 0.00b
		Lower	BJY872D	81.25 ± 10.83abc	10.42 ± 7.22abc	2.08 ± 3.61a	2.08 ± 3.61ab	25.00 ± 22.53de	0.00 ± 0.00b	4.17 ± 7.22c	18.75 ± 22.53a	22.92 ± 13.01a	8.33 ± 9.55ab
	Third time	Up	BJY873U	97.92 ± 3.61a	2.08 ± 3.61c	0.00 ± 0.00a	0.00 ± 0.00b	64.58 ± 23.66abc	2.08 ± 3.61b	0.00 ± 0.00c	0.00 ± 0.00b	18.75 ± 16.54a	12.50 ± 10.83ab
		Middle	BJY873M	97.92 ± 3.61a	0.00 ± 0.00c	0.00 ± 0.00a	2.08 ± 3.61ab	70.83 ± 14.43ab	4.17 ± 3.61ab	14.58 ± 15.73abc	0.00 ± 0.00b	2.08 ± 3.61a	4.17 ± 3.61ab
		Lower	BJY873D	85.42 ± 25.26abc	10.42 ± 18.04abc	0.00 ± 0.00a	0.00 ± 0.00b	37.50 ± 16.54abcde	8.33 ± 14.43ab	6.25 ± 10.83c	0.00 ± 0.00b	20.83 ± 20.09a	4.17 ± 3.61ab
Diseased group	First time	Lower	BJB871D	62.92 ± 18.76bcd	21.42 ± 15.65abc	4.17 ± 3.61a	0.00 ± 0.00b	11.74 ± 6.39de	2.08 ± 3.61b	4.17 ± 7.22c	4.17 ± 7.22ab	12.50 ± 10.83a	2.08 ± 3.61b
	Second time	Up	BJB872U	91.67 ± 7.22ab	4.17 ± 3.61bc	4.17 ± 3.61a	0.00 ± 0.00b	37.75 ± 6.27abcde	3.13 ± 4.42b	29.30 ± 9.59a	0.00 ± 0.00b	3.13 ± 4.42a	3.13 ± 4.42b
		Middle	BJB872M	41.67 ± 46.07d	33.33 ± 34.42a	6.25 ± 6.25a	6.25 ± 6.25a	25.00 ± 38.02de	0.00 ± 0.00b	0.00 ± 0.00c	0.00 ± 0.00b	6.25 ± 6.25a	8.33 ± 9.55ab
		Lower	BJB872D	93.75 ± 6.25ab	2.08 ± 3.61c	2.08 ± 3.61a	2.08 ± 3.61ab	72.92 ± 19.09a	0.00 ± 0.00b	0.00 ± 0.00c	0.00 ± 0.00b	14.58 ± 9.55a	4.17 ± 3.61ab
	Third time	Up	BJB873U	87.50 ± 12.50ab	4.17 ± 3.61bc	6.25 ± 6.25a	0.00 ± 0.00b	2.08 ± 3.61e	22.92 ± 29.54a	10.42 ± 7.22bc	2.08 ± 3.61ab	20.83 ± 21.95a	18.75 ± 18.75a
		Middle	BJB873M	95.83 ± 7.22ab	0.00 ± 0.00c	4.17 ± 7.22a	0.00 ± 0.00b	41.67 ± 26.02abcd	8.33 ± 9.55ab	8.33 ± 3.61bc	10.42 ± 13.01ab	14.58 ± 7.22a	6.25 ± 6.25ab
		Lower	BJB873D	100.00 ± 0.00a	0.00 ± 0.00c	0.00 ± 0.00a	0.00 ± 0.00b	29.17 ± 34.42cde	10.42 ± 13.01ab	25.00 ± 27.24ab	2.08 ± 3.61ab	4.17 ± 3.61a	2.08 ± 3.61b

The data in the table are mean ± SD (n = 3). Different lowercase letters in the same column indicate significant differences in treatments at 0.05 level (p < 0.05).