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. 2022 Jul 28;13:952820. doi: 10.3389/fpls.2022.952820

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Copyright © 2022 Jahan, Yamazaki, Islam, Ghosh, Yoshimura, Kato, Ishizaki, Shinozawa, Sakata and Takezawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ABA-induced expression of LEA-like genes in wild type (WT) and B3-Raf genome editing lines. Quantitative RT-PCR analysis was carried out using RNA isolated from gemmalings treated with or without 10 μM ABA. Transcripts for MpLEAL1 (Mapoly0112s0030) (A), MpLEAL3 (Mapoly0035s0082) (B), MpLEAL5 (Mapoly0087s0015) (C), and MpLEAL6 (Mapoly0027s0114) (D) were analyzed. Relative values were determined by SYBR green-based quantitative PCR analysis using MpEF1 (Mapoly0024s0116) unaffected by ABA as a reference. Error bars indicate +/− SE. *p < 0.05, **p < 0.01 by t-test (n = 3) compared with WT of the same treatment.