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. 2022 Aug 5;27(15):4985. doi: 10.3390/molecules27154985

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of MR extract on SIRTs and claudin-1 expression in the kidneys of diabetic rats. (A) western blotting analysis of SIRT1, SIRT3, SIRT4, and claudin-1 expression. β-Actin expression was used as the loading control. The results represent three independent experiments. (B) Immunohistochemical analysis of SIRT-1 and claudin-1 in the kidneys of mice with STZ-induced diabetes. Original magnification: 200×, scale bar: 50 μm. NC: Normal control, STZ: streptozotocin-treated group, STZ + MR: Streptozotocin-treated rats received MR extract, STZ + Mef: Streptozotocin-treated rats received Metformin.