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. 2022 Aug 8;11(15):2068. doi: 10.3390/plants11152068

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histochemical results obtained from calycinal glands of Rivea ornata. (A) Unstained calycinal glands. (B) Sudan black B test for total lipids. (C) Neutral red fluorochrome test for total lipids. (D) Ferric chloride test for phenolic compounds. (E) Potassium dichromate test for phenolic compounds. (F) Lugol’s iodine test for starch. (G) Periodic acid–Schiff’s reagent (PAS) test for neutral polysaccharides. (H) Ruthenium red test for acidic polysaccharides. (I) Mercuric bromophenol blue test for proteins. (J) Nadi reagent test for terpenoids. (K) Naturstoff reagent A under fluorescent microscopy detecting flavonoids. (L) Dragendorff reagent test for alkaloids. (M) Wagner’s reagent test for alkaloids. (N) UV autofluorescence of calycinal glands. Note: (B–E,G,H,J–M) show positive reactions, while (F,I) show negative reactions. Abbreviations: b, base; h, head; s, stalk. Scale bars 20 μm.