Skip to main content
. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Nature. 2022 Mar 30;604(7904):152–159. doi: 10.1038/s41586-022-04530-6

Extended Data Fig. 12. Local transcription enables NER irrespective of Mfd (extension to Fig. 5).

Extended Data Fig. 12.

a-h, Depriving the genomic loci of transcription drastically diminished NER irrespective of Mfd. Genomic DNA repair within the insulator was monitored as described in Methods for the lesions generated by 4-NQO (a-b), NFZ (c-d), cisplatin (e-f), or UV-C (g-h). lacZ was induced with IPTG followed by the exposure to drugs or UV radiation. Cells were allowed to recover for the indicated time intervals followed by the isolation of genomic DNA. Lesion density was determined by SLR-qPCR and used to calculate the percentage of repaired lesions. Values are the means ± SD (n = 3).