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Cytotoxic activity of the free peptide Ib−M1 (12.5 µM), the bioconjugate Ib−M1/Alg−Chi (12.5 µM) and Alg−Chi NPs (0.4 mg/mL) in Vero cells. Each result was evaluated in triplicate in two independent experiments and expressed in terms of arithmetic average plus standard deviation. Cells maintained in culture medium without any treatment were used as a negative control (— Control; not shown). p < 0.0001: ****; p ≤ 0.0001: ***.
