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Time course of ISPNAP inactivation in NDO-catalyzed reaction mixtures containing benzene. Details of the experimental conditions are described in Materials and Methods. Reaction mixtures contained NADH and all three NDO components in 50 mM MES (pH 6.8) with added benzene (■), benzene and catalase (○), or benzene and Fe(NH4)2(SO4)2 · 6H2O (●) or with no addition (□).
