TABLE 1.

Formation of cis-benzene 1,2-dihydrodiol by NDO^a

Reaction condition	Amt (nmol) of cis-benzene 1,2-di-hydrodiol formed/mlb	Turnoverc
NADH + benzene	0.23	0.45
NADH + benzene + Fe2+	1.31	2.59
NADH + benzene + catalase	0.90	1.78
NADH + benzene + catalase + Fe2+	1.10	2.17
Benzene + Fe2+ + catalase	—d	—

^aReactions were conducted for 1 h. Details of the experimental conditions and product analysis are described in Materials and Methods. 

^bValues are the averages of two different experiments with two different assays. Standard deviations were less than 5% of the values given. 

^cRatio of cis-benzene 1,2-dihydrodiol to the α and β subunits of ISP_NAP. 

^dNot detected.