TABLE 1.
Strains and plasmids included in this study
| K. pneumoniae strain or plasmid | Relevant phenotype | Source or reference |
|---|---|---|
| Porin-sufficient strainsa | ||
| SD8 | OmpK36+ OmpK35+ OmpK37− | 13 |
| CSUB10S | OmpK36+ OmpK35− OmpK37− | 4 |
| Porin-deficient strainsb | ||
| CSUB10R | OmpK36− OmpK35− OmpK37− | 4 |
| LB4 | OmpK36− OmpK35− OmpK37+ | 18 |
| LB66 | OmpK36− OmpK35− OmpK37+ | 15 |
| Plasmids | ||
| pBluescript KS(−) | High-copy-number cloning vector, Apr | Stratagene |
| pGEM-T | PCR fragment cloning vector, Apr | Invitrogen |
| pWSK29 | Low-copy-number cloning vector, Apr | 29 |
| pCSI2 | Kmr cassette-containing vector | 9 |
| pSUV7 | pACYC184 with cloned ompK36 | 3 |
| pSHA25K | pWSK29 with cloned ompK36 gene and Kmr cassette from pCSI2 | This work |
| pSHA16 | pWSK29 with cloned ompK35 | Our laboratory |
| pSHA16K | pWSK29 with cloned ompK35 gene and Kmr cassette from pCSI2 | This work |
| pQE1 | pWSK29 carrying ompK37-containing EcoRI fragment | This work |
| pQE1K | pQE1 with cloned Kmr cassette from pCSI2 | This work |
| pQE3 | pBluescript KS(−) carrying ompK37-containing BamHI fragment from pQE1 | This work |
| pQE3K | pQE3 with cloned Kmr cassette from pCSI2 | This work |
| pQE7 | pWSK29 carrying ompK37 transcribed by lac promoter | This work |
| pQE7K | pQE7 with cloned Kmr cassette from pCSI2 | This work |
| pQE31 | pBluescript KS(−) containing ompK37 and 700 bp of ompK37 upstream flanking sequence | This work |
| pQE31K | pQE31 with cloned Kmr cassette from pCSI2 | |
| pQE33 | pBluescript KS(−) containing ompK37 and 200 bp of ompK37 upstream flanking sequence | This work |
| pQE33K | pQE33 with cloned Kmr cassette from pCSI2 | This work |
a
Porin-sufficient strains are strains expressing either porin OmpK36 or OmpK35 or both.
b
Porin-deficient strain are strains lacking expression of porins OmpK36 and OmpK35.