. 1999 May;181(9):2726–2732. doi: 10.1128/jb.181.9.2726-2732.1999

TABLE 3.

MICs of β-lactam antibiotics for clonally related K. pneumoniae strains expressing different porins

Strain	Plasmid	Porin phenotype^a	Porin expression^b	MIC (μg/ml)^c of:
Strain	Plasmid	Porin phenotype^a	Porin expression^b	CTX	FOX	MP	IP
CSUB10S		OmpK36⁺ OmpK35⁻ OmpK37⁻		4	2	0.06	0.125
CSUB10R		OmpK36⁻ OmpK35⁻ OmpK37⁻		512	128	2	0.5
CSUB10R	pSHA25K	OmpK36⁺ OmpK35⁻ OmpK37⁻	High	4	2	0.06	0.06
CSUB10R	pSHA16K	OmpK36⁻OmpK35⁺ OmpK37⁻	High	4	4	0.12–0.06	0.06
CSUB10R	pQE1K	OmpK36⁻ OmpK35⁻OmpK37⁺	Not detectable	512	64	2	0.25
CSUB10R	pQE3K	OmpK36⁻ OmpK35⁻OmpK37⁺	Low	256	64–32	2	0.25
CSUB10R	pQE7K	OmpK36⁻ OmpK35⁻OmpK37⁺	High	128	32	0.06	0.125

Analyzed by SDS-PAGE and Coomassie blue staining. In strain CSUB10R(pQE1K), OmpK37 expression was detected by Western blot analysis with anti-OmpK37 serum. Plasmid-encoded porins are in boldface.

The level of each expressed porin from the corresponding plasmid was determined by SDS-PAGE analysis and Coomassie blue staining (Fig. 3).

CTX, cefotaxime; FOX, cefoxitin; M, meropenem; IP, imipenem.