TABLE 2.
Isoprenoid levels in rth mutantsa
| Strainc | Level (μg/g [dry wt] of cells)b
|
||||||
|---|---|---|---|---|---|---|---|
| UQ-8 | DMK-8 | MK-8 | C50-P | C55-P | C60-P | Total Pol-P | |
| rth+ | 870 (14) | 157 (3) | 86 (4) | 28 (± 0.2) | 515 (± 18) | 23 (± 0.8) | 566 (± 18) |
| rth-2 | 1,155 (21) | 240 (6) | 99 (4) | 62 (± 5) | 114 (± 38) | 36 (± 12) | 210 (± 55) |
| rth-4 | 1,074 (40) | 236 (4) | 69 (10) | 59 (± 6) | 132 (± 21) | 88 (± 14) | 279 (± 41) |
| rth-6 | 1,131 (19) | 224 (9) | 68 (0.1) | 45 (± 3) | 60 (± 3) | 29 (± 2) | 134 (± 8) |
a
Values shown represent averages of results obtained from two (for polyprenyl phosphates) or more (for isoprenoid quinones) assays. The numbers in parentheses indicate standard deviations (for isoprenoid quinones) or ranges (for polyprenyl phosphates).
b
UQ-8, ubiquinone-8; DMK-8, demethylmenaquinone-8; MK-8, menaquinone-8; C50-P, decaprenyl phosphate; C55-P, undecaprenyl phosphate; C60-P, dodecaprenyl phosphate; Pol-P, polyprenyl phosphate.
c
rth+ indicates the strain MG1655rr/pJK286-rth+ cdsA+. rth-2, rth-4, and rth-6 are its derivatives harboring the respective mutant alleles.