Table 8. Strains, plasmids and relevant information.
| Strain/Plasmid | Relevant Characteristics | Source |
|---|---|---|
| E. amylovora strains | ||
| Ea1189 | Wild Type | [3] |
| Ea1189Δ8 | Deletion of edcA-E and pdeA-C | This study |
| Ea1189Δ12 | Deletion of edcA-E, pdeA-C, EAM_3378, EAM_3136, EAM_2449 and EAM_1579 | [72] |
| Ea1189Δ12ΔfliC | Deletion of fliC in Ea1189Δ12 | This study |
| Ea1189Δ12ΔhofC | Deletion of hofC in Ea1189Δ12 | This study |
| Ea1189ΔfliC | Deletion of fliC in Ea1189 | This study |
| Ea1189ΔhofC | Deletion of hofC in Ea1189 | This study |
| Ea1189Δ12 + edcA-E | Chromosomal restoration of the indicated gene in Ea1189Δ12 | This study |
| Plasmids | ||
| pKD3 | Cmr cassette flanking FRT* sites; Cmr | [61] |
| pKD4 | Kmr cassette flanking FRT sites; Kmr | [61] |
| pKD46 | L-Arabinose-inducible lambda red recombinase; Apr | [61] |
| pTL18 | IPTG-Inducible FLPase, TetR | [73] |
| pBBR1-MCS5 | Broad-host-range cloning vector**; R6K ori; Gmr | [74] |
| pMP2444 | pBBR1MCS-5 expression gfp under lac promoter, Gmr | [75] |
| pEVS143 | Broad-host-range, IPTG inducible (Ptac) cloning vector; inducible Cmr and GFP Kmr | [76] |
| hofC OE | hofC in pEVS143 | This study |
| fliC OE | fliC in pEVS143 | This study |
| edcA OE | edcA in pEVS143 | [3] |
| edcB OE | edcB in pEVS143 | [3] |
| edcC OE | edcC in pEVS143 | [3] |
| edcD OE | edcD in pEVS143 | [3] |
| edcE OE | edcE in pEVS143 | [3] |
*FRT: Flippase target recognition
**MCS: Multiple cloning site.