FIG. 1.

Overexpression and isolation of the tail lysozyme, gp 5. After SDS-PAGE, overexpressed proteins were transferred onto a polyvinylidene difluoride membrane and stained with Coomassie brilliant blue R250 (lanes 1 to 3) or subjected to immunoblotting (lane 4 and 5). Lanes: 1, molecular mass standard; 2 and 4, overexpressed proteins after Ni-NTA column elution; 3 and 5, tail proteins. Arrow, gp 5* (mature gp 5). The proportional staining density of each band reflects the efficiency of transfer to PVDF membrane and does not necessarily represent the stoichemistry.