Sedimentation equilibrium of pre-gp5-his (A) and gp 5* (B). Molecular weights of 194,400 ± 10,410 and 40,700 ± 4,500 for pre-gp5-his and gp 5*, respectively, were obtained, which indicated that the former is a trimer, and the latter is a monomer. Residuals are the difference between the experimental data and the fitted curve and have to be evenly scattered throughout the cell.