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. 2022 Jul 28;13:926765. doi: 10.3389/fimmu.2022.926765

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Copyright © 2022 Jin, Liu, Vallee, Karadjian, Liu and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LNT failed to reduce the burden of T. spiralis in gut microbiota-dysbiosis mice. (A) Mice were administered an antibiotic treatment (AT) and LNT. (B) Adult worms (7 dpi) and (C) muscle larvae (35 dpi) were recovered from mice in each group, and the abundance of T. spiralis was calculated. (D) Periodic acid Schiff (PAS) staining (200×) of duodenal tissues (Scale bars: 50 μm). (E) Immunohistochemistry of duodenal tissues with anti-Muc2 antibody. (F) mRNA level of Muc2. Data are presented as the mean ± sd (n=6). The data shown are representative of three independent experiments. ns, not significant and ***p<0.001 as indicated by the line (one-way ANOVA with Tukey’s post test). These figures are representative of three independent experiments.