FIG. 5.

Wild-type (wt), ΔoxyR::kan, and rpoS::Tn10 bacteria from overnight cultures in M9 minimal medium were diluted in fresh medium and incubated at 37°C with shaking at 150 rpm. At an OD₆₀₀ of 0.2, NaCl was added to half of each culture, to make a final 500 mM solution, and the rest was used as a control. Samples were collected at 15 min after the addition of NaCl and frozen with liquid nitrogen. Total RNA was purified as described in Materials and Methods. The fluorescence signal of each PCR product was compared to that of gapA. Data are from an average of eight MPCR amplifications. Values from treated samples were divided by those from the corresponding control. All genes were analyzed, but only those genes for which statistically significant (P < 0.05) increases were observed are represented. Error bars were estimated from the corresponding SEM. Bacteria carrying the ΔoxyR::kan mutation had undetectable levels of the corresponding mRNA.