TABLE 1.
PCR primer characteristics
| Primera | n-mer | Sequence | PCR fragment size (bp) |
|---|---|---|---|
| oxyR HEX labeled (forward) | 16 | 5′-CGC GAT CAG GCA ATG G-3′ | 129 |
| oxyR (reverse) | 21 | 5′-CAG CGC TGG CAG TAA AGT GAT-3′ | |
| katG (forward) | 21 | 5′-CTG CGT TTT GAT CCT GAG TTC-3′ | 137 |
| katG 6-FAM labeled (reverse) | 19 | 5′-GGC CCG ATG TAG CGA GAT T-3′ | |
| dps 6-FAM labeled (forward) | 22 | 5′-CAA AAC CCC GCT GAA AAG TTA C-3′ | 142 |
| dps (reverse) | 22 | 5′-GAT ATC TGC GGT GTC GTC ATC T-3′ | |
| gorA (forward) | 20 | 5′-GAT GTA TAC CGC CGT CAC CA-3′ | 125 |
| gorA HEX labeled (reverse) | 19 | 5′-AGC CCT GCA ACA TTT CGT C-3′ | |
| ahpCF 6-FAM labeled (forward) | 18 | 5′-CCG CAG GGT ATC ATC CAG-3′ | 128 |
| ahpCF (reverse) | 18 | 5′-TTA GCC GGG CAA ACT TCA-3′ | |
| sodA (forward) | 18 | 5′-CCG ATT ATG GGC CTG GAT-3′ | 121 |
| sodA 6-FAM labeled (reverse) | 17 | 5′-CAA AAC GTG CCG CTG CT-3′ | |
| trxA HEX labeled (forward) | 20 | 5′-TGC GGT CCG TGC AAA ATG AT-3′ | 148 |
| trxA (reverse) | 21 | 5′-AGC AGC AGA GTC GGG ATA CCA-3′ | |
| gapA 6-FAM labeled (forward) | 20 | 5′-GTC GCT GAA GCA ACT GGT CT-3′ | 131 |
| gapA (reverse) | 23 | 5′-AAG TTA GCG CCT TTA ACG AAC AT-3′ |
a
HEX, 6-carboxy-2′-4′-5′-7′-4-7-hexachlorofluorescein; 6-FAM, 6-carboxyfluorescein-N-hydroxysuccinimide ester.