TABLE 2.
Gene expression during growth of wild-type bacteria in LB nutrient mediuma
| Sampling time (h) | OD600 | Mean fluorescence signal ± SEM of PCR productb
|
||||||
|---|---|---|---|---|---|---|---|---|
| oxyR | katG | dps | gorA | ahpCF | sodA | trxA | ||
| 1.5 | 0.31 | 0.31 ± 0.07 (1.5) | 0.79 ± 0.11 (1.0) | 0.10 ± 0.02 (1.0) | 1.92 ± 0.18 (1.3) | 0.43 ± 0.07 (1.0) | 0.18 ± 0.06 (2.0) | 1.19 ± 0.12 (1.5) |
| 2 | 0.58 | 0.21 ± 0.02 (1.0) | 0.99 ± 0.08 (1.3) | 0.18 ± 0.02 (1.8) | 1.48 ± 0.28 (1.0) | 0.47 ± 0.10 (1.1) | 0.17 ± 0.06 (1.9) | 0.82 ± 0.14 (1.0) |
| 3 | 1.54 | 0.60 ± 0.15 (2.9) | 3.42 ± 0.55 (4.3) | 0.41 ± 0.02 (4.1) | 2.48 ± 0.33 (1.7) | 0.49 ± 0.08 (1.1) | 0.09 ± 0.02 (1.0) | 1.02 ± 0.13 (1.2) |
| 5 | 3.17 | 0.66 ± 0.07 (3.1) | 5.81 ± 0.21 (7.4) | 6.22 ± 0.38 (62.2) | 4.28 ± 0.43 (2.9) | 0.65 ± 0.09 (1.5) | 0.11 ± 0.02 (1.2) | 1.72 ± 0.25 (2.1) |
| 7 | 4.40 | 1.09 ± 0.30 (5.2) | 4.07 ± 0.40 (5.2) | 8.65 ± 1.50 (86.5) | 6.24 ± 1.57 (4.2) | 1.49 ± 0.14 (3.5) | 0.50 ± 0.12 (5.6) | 1.29 ± 0.30 (1.6) |
| 12 | 4.86 | 1.18 ± 0.22 (5.6) | 3.48 ± 0.43 (4.4) | 7.55 ± 1.37 (75.5) | 6.78 ± 0.91 (4.6) | 2.72 ± 0.55 (6.3) | 0.69 ± 0.13 (7.7) | 1.45 ± 0.27 (1.8) |
a
Cells from overnight cultures in LB broth were diluted in fresh medium and incubated at 37°C with shaking at 150 rpm. Samples were collected at regular time intervals and rapidly cooled to 0°C. Culture growth at the indicated times was monitored by measuring the OD600. RNA was purified and processed as specified in Materials and Methods.
b
Based on eight MPCR amplifications. Data are relative to the fluorescence signal of the reference gene, gapA. Boldface type indicates statistically significant increments (P < 0.05).