TABLE 2.
Sampling time (h) | OD600 | Mean fluorescence signal ± SEM of PCR productb
|
||||||
---|---|---|---|---|---|---|---|---|
oxyR | katG | dps | gorA | ahpCF | sodA | trxA | ||
1.5 | 0.31 | 0.31 ± 0.07 (1.5) | 0.79 ± 0.11 (1.0) | 0.10 ± 0.02 (1.0) | 1.92 ± 0.18 (1.3) | 0.43 ± 0.07 (1.0) | 0.18 ± 0.06 (2.0) | 1.19 ± 0.12 (1.5) |
2 | 0.58 | 0.21 ± 0.02 (1.0) | 0.99 ± 0.08 (1.3) | 0.18 ± 0.02 (1.8) | 1.48 ± 0.28 (1.0) | 0.47 ± 0.10 (1.1) | 0.17 ± 0.06 (1.9) | 0.82 ± 0.14 (1.0) |
3 | 1.54 | 0.60 ± 0.15 (2.9) | 3.42 ± 0.55 (4.3) | 0.41 ± 0.02 (4.1) | 2.48 ± 0.33 (1.7) | 0.49 ± 0.08 (1.1) | 0.09 ± 0.02 (1.0) | 1.02 ± 0.13 (1.2) |
5 | 3.17 | 0.66 ± 0.07 (3.1) | 5.81 ± 0.21 (7.4) | 6.22 ± 0.38 (62.2) | 4.28 ± 0.43 (2.9) | 0.65 ± 0.09 (1.5) | 0.11 ± 0.02 (1.2) | 1.72 ± 0.25 (2.1) |
7 | 4.40 | 1.09 ± 0.30 (5.2) | 4.07 ± 0.40 (5.2) | 8.65 ± 1.50 (86.5) | 6.24 ± 1.57 (4.2) | 1.49 ± 0.14 (3.5) | 0.50 ± 0.12 (5.6) | 1.29 ± 0.30 (1.6) |
12 | 4.86 | 1.18 ± 0.22 (5.6) | 3.48 ± 0.43 (4.4) | 7.55 ± 1.37 (75.5) | 6.78 ± 0.91 (4.6) | 2.72 ± 0.55 (6.3) | 0.69 ± 0.13 (7.7) | 1.45 ± 0.27 (1.8) |
Cells from overnight cultures in LB broth were diluted in fresh medium and incubated at 37°C with shaking at 150 rpm. Samples were collected at regular time intervals and rapidly cooled to 0°C. Culture growth at the indicated times was monitored by measuring the OD600. RNA was purified and processed as specified in Materials and Methods.
Based on eight MPCR amplifications. Data are relative to the fluorescence signal of the reference gene, gapA. Boldface type indicates statistically significant increments (P < 0.05).