Figure 1. Placental villus structure and specificity of markers used to exclude unwanted cell populations.

(A) Placental villous morphology and plane of section, (B) haematoxylin and eosin staining of a thin section though a placental villus (7.1 weeks), localisation of (C) β4 integrin (red) to cytotrophoblasts and (E) CD144 (red) and CD31 (green) to blood vessels (white arrows) in placental villus sections confirmed antibody specificity. No fluorescence is seen in negative IgG controls (D, F) run simultaneously. Nuclei are counterstained with DAPI (blue). Scale bar = 100 µm. Rendered images in (A) have been acquired from Biorender.com.