Figure 4. In vitro culture of CD73⁺CD90⁺ and podoplanin⁺CD36⁺ cells.

(A) FACS sorting was used to isolate CD73⁺CD90⁺ and podoplanin⁺CD36⁺ cells from placental villous core cells (n=3). (B) Morphology and phenotype of CD73⁺CD90⁺ cells after 7 days in culture. (C) Phenotype of podoplanin⁺CD36⁺ cells after 7 days in culture. (D) CD146 and CD142 expression on CD73⁺CD90⁺ analysed with Panel One (day 0, n=5) or at 7 days after culture (n=3). (E) CD146 and CD142 expression on podoplanin⁺CD36⁺ cells analysed with Panel One (day 0, n=5) or at 7 days after culture (n=3). (F) Isolation of explant-derived pMSCs, and morphology and phenotype of passaged pMSCs (n=3). Error bars = standard error of the mean and scale bar = 100 µm.

Figure 4—figure supplement 1. Representative 2-dimensional flow cytometry plots displaying the phenotype of FACS sorted CD73⁺CD90⁺ and podoplanin⁺CD36⁺ cells after 7 days culture in vitro (n=3), and explant isolated pMSCs after culture in vitro (n=3, p2-6).

Figure 4—figure supplement 2. Flow cytometry histograms displaying the phenotype of FACS sorted CD73⁺CD90⁺ and podoplanin⁺CD36⁺ cells after 7 days culture in vitro (n=3), and explant isolated pMSCs after culture in vitro (n=3, p2-6).