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. 2022 Aug 3;11:e76622. doi: 10.7554/eLife.76622

Table 3. Panel One designed to assess villous core cells on a 3 L Cytek Aurora.

Table 3—source data 1. All antibodies used in Panel One were titrated on placental villous core digest cells.
Table 3—source data 2. Titration of additional antibodies, not contained in Panel One, required for the FACS sorting with Panel Two.
Table 3—source data 3. Representative images depicting how forward scatter (FSC) (A) or addition of cell-specific antibodies improved detection of appropriate doses for specific placental populations.
(B) β4 integrin+ cells were negative for podoplanin but demonstrated an unspecific shift in expression at higher doses. (C) Removal of β4 integrin+ improved detection of the optimal podoplanin dose.
Antibody Fluorophore Clone Flow cytometry Dose (μL) Reference control Supplier
CD55 BB515 IA10 0.6 Villous core cells Beckman Coulter
β4 integrin FITC 450-9D 0.6 Villous core cells Thermofisher
CD34 PerCP 581 0.3 Villous core cells BD
CD36 PerCpVio700 REA760 0.6 Villous core cells Miltenyi Biotec
VEGFR2 PE 7D4-6 0.6 Villous core cells Biolegend
CD271 PE-CF594 C40-1457 0.6 Stromal vascular fraction Biolegend
CD142 BB700 HTF-1 1.25 Villous core cells BD
CD26 PE/Cy5 BA5b 0.15 Villous core cells BD
PDPN PE/Cy7 NC-08 0.3 Villous core cells BD
CD248 Alexa Fluor 647 B1/35 0.6 Stromal vascular fraction BD
CD41 APC HIP8 0.3 Beads BD
CD90 Alexa700 5E10 0.6 Villous core cells Biolegend
CD39 BUV737 TU66 1.25 Beads BD
ICAM1 APC/Fire750 HA58 2.5 Stromal vascular fraction Biolegend
CD144 BV421 55–7 H1 1.25 Villous core cells BD
CD235a Pacific Blue H1264 0.3 Villous core cells BD
CD31 BV480 WM59 0.3 Villous core cells BD
CD45 Krome-Orange B61840 0.3 Villous core cells Beckman Coulter
CD146 BV605 PIH12 0.3 Villous core cells BD
CD117 BV650 104D2 0.3 Beads BD
HLADR BV750 L243 0.3 Villous core cells BD
CD73 BV785 AD2 0.3 Villous core cells BD