FIG. 5.

Effect of increasing the length of 3′CIS, by adding 1 to 9 nt at map position 1912, on the copy number of the IncB replicon of pMU1598 and pMU1599. The copy number was determined by assaying the CAT activity of E. coli JP3438 carrying pMU1598, pMU1599, or their derivatives in the absence of IPTG and is expressed relative to the value obtained for the parental plasmid (i.e., either pMU1598 or pMU1599, as appropriate). The values shown are the averages (± standard deviations) of at least six independent determinations. WT, wild type.