TABLE 1.
Plasmids
| Plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| pMU720 | Miniplasmid derived from pMU707; Gal IncB | 8 |
| pMU604 | Miniplasmid derived from pMU407.1; Gal IncL/M | 12 |
| pMU605 | Miniplasmid derived from R64-11; Gal IncI1 | 12 |
| pMU1531 | Miniplasmid derived from R144-3; Gal Inc IncI1 | 29 |
| pMU1530 | Miniplasmid derived from R621a; Gal Inc IncIγ | 29 |
| pMU2200 | Miniplasmid derived from pIE545; Gal IncZ | 34 |
| pMU2209 | Miniplasmid derived from R387; Gal IncK | 34 |
| pMU1597 | pBR322 derivative carrying a repA-lacZ transcriptional fusion containing nt 1–789 of pMU720; Ap IncB | This study |
| pMU1598 | pAM34 carrying nt 1–2170 of pMU720 with a T642C copy up mutation; Ap Cm lacIq IncB | This study |
| pMU1599 | pAM34 carrying nt 1–2170 of pMU720; Ap Cm lacIq IncB | This study |
| pACYC184 | p15A replicon; Tc Cm | 10 |
| pAM34 | pMB1 derivative in which the preprimer RNA is expressed from lacZpo; lacIq Ap Sp | 15 |
a
Ap, ampicillin resistance; Cm, chloramphenicol resistance; Tc, tetracycline resistance; Sp, spectinomycin resistance. Mutations introduced into plasmids pMU1598 and pMU1599 are described in Results.