Fig. 5.

OX skin inflammation impairs lymph flow in conduits. (A) Conduit (red) in control and OXd4 iLNs (Left). The density (Center) and the diameter (Right) of collagen I⁺ conduits in the iLNs of control and OXd4 mice. n = 4 to 5 per group. (B) The schematic diagram of the experimental design. Mice were treated with OX on their shaved abdomens. The control and OXd4 mice were treated with FITC on both flanks. After 2 or 24 h, the iLNs were collected for analysis. (C) FITC distribution with conduits in the iLNs at 2 h after FITC sensitization. LN cryosections were stained with anti-ERTR7 (red; FRCs) and anti-collagen I (blue; conduit). (D) FITC distribution (Left) and density (Right) at 24 h after FITC sensitization. Scale bar = 200 μm. (E) 3D-reconstructed images of FITC in whole-mount LN at 24 h after FITC sensitization (Left). FITC density with depth was quantified every 30 μm from the capsule (Right). n > 5 mice per group. (F–I). Control and OXd4 LNs were collected at 2 and 24 h after FITC sensitization. (F) Representative flow cytometry plot of FITC⁺ DCs. (G–I) FITC⁺cDC2, cDC1, and SCS macrophage quantification. n = 10 iLNs/group. (A, D, E, and G–I) Data are mean ± SEM (unpaired one-way ANOVA [A and G–I] or unpaired Student’s t test [D and E]). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0005; ns, no significance.